Reduced translation efficiency due to novel splicing variants in 5′ untranslated region and identification of novel cis-regulatory elements in canine and human BRCA2

Abstract Background Breast cancer 2, early onset ( BRCA2 ) is a tumor suppressor gene. The protein encoded by this gene plays an important role in homologous recombination (HR)-mediated DNA repair. Deleterious mutations and downregulation of its expression have been associated with tumorigenesis dogs humans. Thus, regulation level for maintaining homeostasis recombination. Results In study, the mechanisms that regulate were proposed. Novel splicing variants identified 5′ untranslated region (UTR) canine human testis, ovary, cultured cell lines. cells, ratio at UTR was altered serum starvation. These novel variants, excluding one found to reduce translational efficiency. Additionally, sequence intron 1 harbored cis-regulatory elements. Three silencer two enhancer elements 1. Conclusions This study demonstrates regulated via harbors